LyteStar Rickettsial Diseases PCR Kit

For detection of Pan-Rickettsia and Pan-Orientia from human specimens

The LyteStar™ Rickettsial Diseases PCR Kit 1.0 is intended for the specific detection and differentiation of pan-Rickettsia and pan-Orientia DNA in human whole blood, serum and eschar tissues (biopsy or swab), for the diagnosis of spotted fever, typhus fever and scrub typhus infections. The LyteStar™ Rickettsial Diseases PCR Kit 1.0 is a dual-target assay comprising screening assays targeting the pan-Orientia 16S rRNA (rrs) gene and the pan-Rickettsia citrate synthase (gltA) gene.

Specifications and Features

Multiplex kit for Detection and Differentiation:
Highest Analytical Sensitivity
Pan-Orientia specific; rrs-gene
2.23 cp/μl
Pan-Rickettsia specific; gltA-gene
5.56 cp/μl
Internal Control (IC); heterologous RNA

100% Analytical Specificity

  • The specificity of the kit was evaluated by testing genomic RNA/DNA extracted from other pathogens likely to be present in the same sample material as pan-Orientia and pan-Rickettsia, or that cause similar symptoms to these bacteria.
  • No cross reactivity was observed with other pathogens or genotype/subtype, other than the intended target.

Integrated Quality Monitoring

  • The kit includes a heterologous amplification system (Internal Control; IC) to identify possible PCR inhibition and to confirm the integrity of the reagents of the kit. The IC may also be used to monitor nucleic acid extraction efficiency.
  • A Positive Control (PC) with in vitro transcripts of synthesized target genes of pan-Orientia and pan-Rickettsia confirms the integrity and performance of the kit components.
  • A Negative Control (PCR-grade water) monitors potential contamination and excludes false positive results.

Convenience and Efficiency

  • Harmonized cycling profiles with selected other AstronDX products to perform analysis of multiple targets concurrently in the same PCR run.
  • Validated for use with various extraction kits and PCR cyclers – Please always refer to latest Instructions for Use (IFU).
  • 96 rxn and 24 rxn kit sizes accommodate various throughput scenarios, with Master reagents being provided in multiple tubes to reduce freeze-thaw cycles.
  • Ready-to-use reagents and an intuitive, uncomplicated pipetting scheme to minimize errors and guarantee efficient workflows and routine procedures.

Order Information

Product Name
LyteStar Rickettsial Diseases PCR Kit 1.0

Cat. No.     /     Reactions
887103                   96
887101                   24

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Information

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    ​Selangor, Malaysia
  • +603 7931 6760
  • info@astrondx.com

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