LyteStar MERS-CoV RT-PCR Kit

For detection of Middle East Respiratory Syndrom Coronavirus (MERS-CoV) from human specimens

The LyteStar™ MERS-CoV RT-PCR Kit 1.0 is intended for the specific detection of MERS-CoV RNA in human respiratory specimens (bronchoalveolar lavage, tracheal aspirate, sputum, nasopharyngeal and oropharyngeal swabs placed in VTM, and nasopharyngeal wash/aspirate). The LyteStar™ MERS-CoV RT-PCR Kit 1.0 is a dual target assay comprising a screening assay targeting the upE gene and a confirmatory assay targeting the Orf1a gene. 

Specifications and Features

Multiplex kit for Detection and Differentiation:
Highest Analytical Sensitivity
Screening; upE-gene
1.45 cp/μl
Confirmatory; Orf1a-gene
1.49 cp/μl
Internal Control (IC); heterologous RNA

100% Analytical Specificity

  • The specificity of the kit was evaluated by testing genomic RNA/DNA extracted from other pathogens likely to be present in the same sample material as MERS-CoV virus, or that cause similar symptoms to the MERS-CoV virus.
  • No cross reactivity was observed with other human respiratory viruses and other pathogens, other than the intended target.

Integrated Quality Monitoring

  • The kit includes a heterologous amplification system (Internal Control; IC) to identify possible RT-PCR inhibition and to confirm the integrity of the reagents of the kit. The IC may also be used to monitor nucleic acid extraction efficiency.
  • A Positive Control (PC) with in vitro transcripts of synthesized target genes of MERS-CoV confirms the integrity and performance of the kit components.
  • A Negative Control (PCR-grade water) monitors potential contamination and excludes false positive results.

Convenience and Efficiency

  • Harmonized cycling profiles with selected other AstronDX products to perform analysis of multiple targets concurrently in the same PCR run.
  • Validated for use with various extraction kits and PCR cyclers – Please always refer to latest Instructions for Use (IFU).
  • 96 rxn and 24 rxn kit sizes accommodate various throughput scenarios, with Master reagents being provided in multiple tubes to reduce freeze-thaw cycles.
  • Ready-to-use reagents and an intuitive, uncomplicated pipetting scheme to minimize errors and guarantee efficient workflows and routine procedures.

Order Information

Product Name
LyteStar MERS-CoV RT-PCR Kit 1.0

Cat. No.     /     Reactions
889103                   96
889101                   24

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Information

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  • Unit 307, Block B, Phileo Damansara 1,
    ​9, Jalan 16/11, 46350 Petaling Jaya,
    ​Selangor, Malaysia
  • +603 7931 6760
  • info@astrondx.com

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