LyteStar Influenza Typing RT-PCR Kit

For detection of Influenza A and Influenza B virus and typing of Influenza A(H1N1)pdm09 and H3N2 from human specimens

The LyteStar™ Influenza Typing RT-PCR Kit 1.0 is intended for the specific detection of Influenza A/B RNA in human respiratory specimens. The LyteStar™ Influenza Typing RT-PCR Kit 1.0 is a four-target assay comprising a screening assay targeting the Influenza A matrix (M) and Influenza B hemagglutinin (HA) genes. The assay further differentiates the Influenza A into A(H1N1)pdm09 and H3N2, via the HA gene.

Specifications and Features

Multiplex kit for Detection and Differentiation:
Highest Analytical Sensitivity
Influenza A specific; M-gene
0.28 cp/μl
Influenza B specific; HA-gene
0.41 cp/μl
Influenza A(H1N1)pdm09 specific; HA-gene
0.96 cp/μl
Influenza A/H3N2 specific; HA-gene
1.61 cp/μl
Internal Control (IC); heterologous RNA

100% Analytical Specificity

  • The specificity of the kit was evaluated by testing genomic RNA/DNA extracted from other pathogens likely to be present in the same sample material as Influenza virus, or that cause similar symptoms to the Influenza virus.
  • No cross reactivity was observed with other respiratory viruses and other pathogens, other than the intended target.

Clinically Validated

  • The clinical performance of the kit was evaluated through retrospective studies at various public health laboratories in comparison to the clinical site’s reference method.
  • The LyteStar™ Influenza Typing RT-PCR Kit 1.0 achieved 100% sensitivity and 96.77% specificity for the detection of Influenza A, Influenza B, Influenza A(H1N1)pdm09, and Influenza A(H3N2).

Integrated Quality Monitoring

  • The kit includes a heterologous amplification system (Internal Control; IC) to identify possible RT-PCR inhibition and to confirm the integrity of the reagents of the kit. The IC may also be used to monitor nucleic acid extraction efficiency.
  • A Positive Control (PC) with in vitro transcripts of synthesized target genes of Influenza A, Influenza B, Influenza A(H1N1)pdm09 and Influenza A(H3N2) confirms the integrity and performance of the kit components.
  • A Negative Control (PCR-grade water) monitors potential contamination and excludes false positive results.

Convenience and Efficiency

  • Harmonized cycling profiles with selected other AstronDX products to perform analysis of multiple targets concurrently in the same PCR run.
  • Validated for use with various extraction kits and PCR cyclers – Please always refer to latest Instructions for Use (IFU).
  • 96 rxn and 24 rxn kit sizes accommodate various throughput scenarios, with Master reagents being provided in multiple tubes to reduce freeze-thaw cycles.
  • Ready-to-use reagents and an intuitive, uncomplicated pipetting scheme to minimize errors and guarantee efficient workflows and routine procedures.

Order Information

Product Name
LyteStar Influenza Typing RT-PCR Kit 1.0

Cat. No.     /     Reactions
890003                   96
890001                   24

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Information

AstronDX Technologies Sdn Bhd

  • Unit 307, Block B, Phileo Damansara 1,
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    ​Selangor, Malaysia
  • +603 7931 6760
  • info@astrondx.com

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