LyteStar COVID/ Flu/ RSV Differentiation RT-PCR Kit 1.0

LyteStar COVID/ Flu/ RSV Differentiation RT-PCR Kit

For detection of SARS-CoV-2, Influenza A, Influenza B and Respiratory Syncytial Virus from human specimens

The LyteStar™ COVID/ Flu/ RSV Differentiation RT-PCR Kit 1.0 is intended for the specific detection of SARS-CoV-2, Influenza A, Influenza B and Respiratory Syncytial Virus (RSV) RNA in human respiratory specimens. The LyteStar™ COVID/ Flu/ RSV Differentiation RT-PCR Kit 1.0 is a four-target assay comprising of detection and differentiation assays targeting the SARS-CoV-2 specific RNA-dependent RNA polymerase (RdRP) gene, the Influenza A specific Matrix (M) gene, the Influenza B specific Haemagglutinin (HA) gene and the RSV specific Matrix (M) gene.

Specifications and Features

Multiplex kit for Detection and Differentiation:	Highest Analytical Sensitivity
SARS-CoV-2 specific; RdRP-gene	0.31 cp/μl
Influenza A specific; M-gene	0.82 cp/μl
Influenza B specific; HA-gene	0.85 cp/μl
RSV specific; M-gene	1.53 cp/μl
Internal Control (IC); heterologous RNA

100% Analytical Specificity

The specificity of the kit was evaluated by testing genomic RNA/DNA extracted from other pathogens likely to be present in the same sample material as SARS-CoV-2, Influenza A, Influenza B and RSV, or that cause similar symptoms to these viruses.
No cross reactivity was observed with other human respiratory viruses and other pathogens, other than the intended target.

Clinically Validated

The clinical performance of the kit was evaluated through retrospective studies at various hospitals and public health laboratories in comparison to the clinical site’s reference method.
The LyteStar™ COVID/ Flu/ RSV Differentiation RT-PCR Kit 1.0 achieved 100% sensitivity for the detection of SARS-CoV-2, Influenza A, Influenza B and RSV, and 100% specificity for the detection of SARS-CoV-2 and Influenza B, 99.2% for the detection of Influenza A, and 98.2% for the detection of RSV.

Integrated Quality Monitoring

The kit includes a heterologous amplification system (Internal Control; IC) to identify possible RT-PCR inhibition and to confirm the integrity of the reagents of the kit. The IC may also be used to monitor nucleic acid extraction efficiency.
A Positive Control (PC) with in vitro transcripts of synthesized target genes of SARS-CoV-2, Influenza A, Influenza B and RSV confirms the integrity and performance of the kit components.
A Negative Control (PCR-grade water) monitors potential contamination and excludes false positive results.

Convenience and Efficiency

Harmonized cycling profiles with selected other AstronDX products to perform analysis of multiple targets concurrently in the same PCR run.
Validated for use with various extraction kits and PCR cyclers – Please always refer to latest Instructions for Use (IFU).
96 rxn and 24 rxn kit sizes accommodate various throughput scenarios, with Master reagents being provided in multiple tubes to reduce freeze-thaw cycles.
Ready-to-use reagents and an intuitive, uncomplicated pipetting scheme to minimize errors and guarantee efficient workflows and routine procedures.

Order Information

Product Name
LyteStar COVID/ Flu/ RSV Differentiation RT-PCR Kit 1.0

Cat. No.     /     Reactions
881203      96
881201         24