LyteStar 2019-nCoV RT-PCR Kit

For detection of novel coronavirus (SARS-CoV-2) from human specimens

The LyteStar™ 2019-nCoV RT-PCR Kit 2.0 is intended for the specific detection of SARS-CoV-2 RNA in human respiratory specimens (bronchoalveolar lavage, tracheal aspirate, sputum, nasopharyngeal and oropharyngeal swabs placed in VTM, nasopharyngeal wash/aspirate, and nasal wash/aspirate). The LyteStar™ 2019-nCoV RT-PCR Kit 2.0 is a dual target assay comprising a screening assay targeting the E gene and a confirmation assay targeting the RdRP gene.

Specifications and Features

Multiplex kit for Detection and Differentiation:
Highest Analytical Sensitivity
SARS-CoV related CoV; E-gene
2.14 cp/μl
SARS-CoV-2 specific; RdRP-gene
1.68 cp/μl
Internal Control (IC); heterologous RNA

100% Analytical Specificity

  • The specificity of the kit was evaluated by testing genomic RNA/DNA extracted from other pathogens likely to be present in the same sample material as SARS-CoV-2 virus, or that cause similar symptoms to the SARS-CoV-2 virus.
  • No cross reactivity was observed with other respiratory viruses and other pathogens, other than the intended target.

Clinically Validated

  • The clinical performance of the kit was evaluated through a retrospective study at a public medical university in comparison to the clinical site’s reference method.
  • In comparison to the reference test, all samples tested with 100% concordance.
  • The LyteStar™ SARS-CoV-2 RT-PCR Kit 1.0 achieved 100% sensitivity and 100% specificity for the detection of SARS-CoV-2.

Integrated Quality Monitoring

  • The kit includes a heterologous amplification system (Internal Control; IC) to identify possible RT-PCR inhibition and to confirm the integrity of the reagents of the kit. The IC may also be used to monitor nucleic acid extraction efficiency.
  • A Positive Control (PC) with in vitro transcripts of synthesized target genes of SARS-CoV-2 confirms the integrity and performance of the kit components.
  • A Negative Control (PCR-grade water) monitors potential contamination and excludes false positive results.

Convenience and Efficiency

  • Harmonized cycling profiles with selected other AstronDX products to perform analysis of multiple targets concurrently in the same PCR run.
  • Validated for use with various extraction kits and PCR cyclers – Please always refer to latest Instructions for Use (IFU).
  • 96 rxn and 24 rxn kit sizes accommodate various throughput scenarios, with Master reagents being provided in multiple tubes to reduce freeze-thaw cycles.
  • Ready-to-use reagents and an intuitive, uncomplicated pipetting scheme to minimize errors and guarantee efficient workflows and routine procedures.

Order Information

Product Name
LyteStar 2019-nCoV RT-PCR Kit 2.0

Cat. No.     /     Reactions
888003                   96
888001                   24

All Products

Information

AstronDX Technologies Sdn Bhd

  • Unit 307, Block B, Phileo Damansara 1,
    ​9, Jalan 16/11, 46350 Petaling Jaya,
    ​Selangor, Malaysia
  • +603 7931 6760
  • info@astrondx.com

©Copyright 2025 AstronDX Technologies Sdn Bhd 200901035495 (0878612H) | By Web Design Malaysia, Zoewebs

Facebook Twitter Youtube